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No, there is no limit on the size of the USB drive you can use, however, please ensure it is correctly formatted following the reference below taken from the user manual:

B115/112MP3:
Connector       USB Type A
File System     FAT 16, FAT 32
Format            MP3 (CBR & VBR)
Bit Rates         32 – 320 kbps
Sample Rates  32kHz, 44,1kHz, 48kHz

EPS500MP3/B207MP3/PMP500MP3:
Connector       USB Type A
File System     FAT 16, FAT 32
Format            MP3
Bit Rates         8 – 320 kbps VBR
Sample Rates  8kHz, 16kHz, 11.025kHz, 22.05kHz, 12kHz, 24kHz, 32kHz, 44,1kHz, 48kHz